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These questions are integral to understanding Testosterone and its role in the human body. For thousands of years, since the beginning of civilization, it was clearly understood that the testicles played some kind of key role in the maintenance and development of the male gender and male characteristics. Although its specific role or cause was unknown to humans until approximately 130 years ago, it was understood that there was something about them that contributed to strength, virility, masculinity, and male traits in general. This could not only be observed in humans, but in animals as well. Although there are subtle differences in animal behaviour between the sexes, there are distinct differences between females and males, as well as a distinct difference between the developmental period before and after puberty (which has been linked to observable changes in testicular size and function). Furthermore, it has been observed by many societies and cultures over the thousands of years that the removal of the testicles resulted in the removal of most or all of these typical male characteristics: loss of strength, loss of muscle mass, reduced drive and aggression, etc. Even the ancient Greek and Roman Olympic athletes would consume extreme quantities of sheep and bull testicles, as they observed a significant increase in performance when engaged in this regimen. It was in the 1930s when the core component/ingredient that originated in the testicles, which was responsible for all of these traits was discovered: Testosterone, equipoise high blood pressure. Adolf Butenandt identified, isolated, and synthesized Testosterone in the early 1930s, which would forever change many aspects of medicine, and of course athletics. Following the isolation and identification of Testosterone, research and testing continued in order to manipulate the properties of the hormone. Upon its first isolation, the very first preparations of Testosterone created (Testosterone suspension) were crude and impractical for regular use. Testosterone on its own, pure and unmodified in any way, expressed properties that were too inconvenient and impractical for regular clinical, medical, or even athletic use. First of all, being that it is not very soluble in oil, it had to be suspended in water within microcrystals. Such an injection preparation, known as Testosterone suspension, was far too painful to inject on a regular basis. Furthermore, the half-life of pure Testosterone is approximately 6 ‘ 12 hours, which necessitated either daily or multiple times per day injections. It was not long afterwards during the 1930s that it was discovered that different carboxylic acids could be bonded (via ester bonding) onto the 17-beta hydroxyl group of the steroid structure, which would extend the half-life and release rate of the hormone. The shorter the ester bond, the shorter the half-life is granted to the hormone. The longer the ester bond, the longer the half-life. One of the first esterified formats of Testosterone (also known as a Testosterone ester) was Testosterone Propionate, a fast acting variant that exhibits a half-life of 4. Although this was much longer than pure Testosterone’s half-life, it was still not very practical for medical use. Testosterone Propionate, however, has gained much popularity among athletes and bodybuilders due to the fact that because it is a fast acting variant of Testosterone, blood plasma levels build up in the body quite quickly, resulting in a quicker onset of physique and performance changes and effects. Schering AG was the first company to market and release Testosterone Propionate onto the prescription market. It is still in wide use today by bodybuilders and athletes, though it is the least popular variant. There also exists Testosterone Enanthate and Testosterone Cypionate, two long-acting Testosterone esters that each possess half-lives of 7 ‘ 10 days and 10 ‘ 12 days respectively. The two are almost considered the exact same due to the very similar half-lives.
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