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The results showed that ErGPCR-2 was expressed in the midgut, fat body and epidermis. In these three tissues, ErGPCR-2 protein and mRNA exhibited higher expression levels at the fifth instar molting (5M) and metamorphic stages (sixth instar 72 h to 120 h) than at the fifth instar feeding (5 h to 24 h) and sixth instar feeding stages from 6–0 h to 6–48 h (Figures 1A, 1B and 1C). The expression level of ErGPCR-2 was upregulated by 20E injection into the sixth instar 6 h larvae but was unaffected by JH III or Dimethyl sulfoxide DMSO injection (Figures 1D and 1E). These results indicate that ErGPCR-2 expression is upregulated by 20E during molting and metamorphosis, cypionex 250. Halotestin culturismo Off-target effect was excluded by examination of another GPCR named ErGPCR-1 (Supplement Files: Figures S2A and B), cypionex 250.
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